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Mass spectrometry of Escherichia coli RNA polymerase: interactions of the core enzyme with sigma70 and Rsd protein.

Abstract:

The E. coli RNA polymerase core enzyme is a multisubunit complex of 388,981 Da. To initiate transcription at promoters, the core enzyme associates with a sigma subunit to form holo RNA polymerase. Here we have used nanoflow electrospray mass spectrometry, coupled with tandem mass spectrometry, to probe the interaction of the RNA polymerase core enzyme with the most abundant sigma factor, sigma70. The results show remarkably well-resolved spectra for both the core and holo RNA polymerases. The...

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Publication status:
Published

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Journal:
Structure (London, England : 1993)
Volume:
12
Issue:
2
Pages:
269-275
Publication date:
2004-02-05
DOI:
EISSN:
1878-4186
ISSN:
0969-2126
URN:
uuid:4e2bc517-b45b-4b7e-a15d-6d71ff808b9a
Source identifiers:
59255
Local pid:
pubs:59255

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