Construction of a YAC contig and an STS map spanning 3.6 megabase pairs in Xp22.1.

Journal article

We have constructed a 3.6 Mb sequence tagged sites (STS)-based yeast artificial chromosome (YAC) contig, consisting of 58 individual YAC clones, spanning the region PDHA1 and DXS451 on Xp22.1. In addition to establishing the order of PDHA1, ISPK-1, DXS2504, DXS1528 and the 13 known polymorphic loci as Xpter-PDHA1-DXS443-DXS3424-ISPK-1-DXS12 29-DXS2504-DXS1528-DXS365-DXS7101- DXS1683-DXS1052-DXS274-DXS92-DXS1226-DX S41-DXS989-DXS451-Xcen, we have also developed 35 novel STSs from YAC end clone...

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Authors: Trump, D; Pilia, G; Dixon, P; Wooding, C; Thakrar, R

• Publication status: Published
• Journal: Human genetics
• Volume: 97
• Issue: 1
• Pages: 60-68
• Publication date: 1996-01-01
• DOI: 10.1007/bf00218834
• EISSN: 1432-1203
• ISSN: 0340-6717
• Language: English
• Keywords: DNA Primers; Base Sequence; X Chromosome; Genetic Markers; Humans; Chromosomes, Artificial, Yeast; Sequence Tagged Sites; Molecular Sequence Data; Restriction Mapping; Base Composition; Chromosome Mapping; Polymerase Chain Reaction