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Amine landscaping to maximize protein-dye fluorescence and ultrastable protein-ligand interaction

Abstract:

Chemical modification of proteins provides great opportunities to control and visualize living systems. The most common way to modify proteins is reaction of their abundant amines with N-hydroxysuccinimide (NHS) esters. Here we explore the impact of amine number and positioning on protein-conjugate behavior using streptavidin-biotin, a central tool for biological research. Dye-NHS modification of streptavidin severely damaged ligand binding. We generated a streptavidin variant retaining ultra...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.chembiol.2017.06.015

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Department:
Oxford, MSD, Biochemistry
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Author
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Department:
Oxford, MSD, Biochemistry
Role:
Author
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Department:
Worcester College
Role:
Author
Publisher:
Cell Press Publisher's website
Journal:
Cell Chemical Biology Journal website
Volume:
24
Issue:
8
Pages:
1040–1047
Publication date:
2017-07-27
Acceptance date:
2017-05-24
DOI:
ISSN:
2451-9456
Pubs id:
pubs:698458
URN:
uri:4dac912b-29f5-4db5-830b-f51bdbbbc1c9
UUID:
uuid:4dac912b-29f5-4db5-830b-f51bdbbbc1c9
Local pid:
pubs:698458
Paper number:
8

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