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Stable and noncompetitive RNA internal control for routine clinical diagnostic reverse transcription-PCR.

Abstract:

Clinical diagnostic tests based on nucleic acid amplification assist with the prompt diagnosis of microbial infections because of their speeds and extremely low limits of detection. However, the design of appropriate internal controls for such assays has proven difficult. We describe a reaction-specific RNA internal control for diagnostic reverse transcription (RT)-PCR which allows extraction, RT, amplification, and detection to be monitored. The control consists of a G+C-rich (60%) RNA molec...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Experimental Medicine Division, Biomedical Research Centre
Role:
Author
Journal:
Journal of clinical microbiology
Volume:
42
Issue:
3
Pages:
1003-1011
Publication date:
2004-03-05
DOI:
EISSN:
1098-660X
ISSN:
0095-1137
URN:
uuid:4d2e6810-417e-4d6c-91a8-59d0eb3dcadd
Source identifiers:
66439
Local pid:
pubs:66439

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