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Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors

Abstract:

Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step ...

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Journal:
Methods in Molecular Biology
Volume:
746
Pages:
297-316
Publication date:
2011-01-01
DOI:
ISSN:
1064-3745
URN:
uuid:4cda282d-fd1b-4f8a-b2f8-3f938748edf7
Source identifiers:
412088
Local pid:
pubs:412088

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