- Abstract:
-
Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step ...
Expand abstract - Publisher copy:
- 10.1007/978-1-61779-126-0-16
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- Journal:
- Methods in Molecular Biology
- Volume:
- 746
- Pages:
- 297-316
- Publication date:
- 2011-01-01
- DOI:
- ISSN:
-
1064-3745
- URN:
-
uuid:4cda282d-fd1b-4f8a-b2f8-3f938748edf7
- Source identifiers:
-
412088
- Local pid:
- pubs:412088
- Copyright date:
- 2011
Journal article
Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors
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