Journal article
Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.
- Abstract:
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Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required a...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 1.2MB)
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- Publisher copy:
- 10.1016/j.dib.2016.06.033
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Authors
Funding
Cancer Research UK
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Danish Cancer Society
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Cambridge University
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Prostate Cancer UK
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Norwegian Cancer Society
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Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Data in Brief Journal website
- Volume:
- 8
- Pages:
- 733-740
- Publication date:
- 2016-06-01
- Acceptance date:
- 2016-06-21
- DOI:
- EISSN:
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2352-3409
- ISSN:
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2352-3409
- Pmid:
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27508226
- Source identifiers:
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817476
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:817476
- UUID:
-
uuid:4c515941-c921-4c60-8fdc-0f4317f45aa7
- Local pid:
- pubs:817476
- Deposit date:
- 2018-01-11
Terms of use
- Copyright holder:
- Crown Copyright
- Copyright date:
- 2016
- Notes:
- Crown Copyright © 2016 Published by Elsevier Inc. This is an open access article under the CCBY license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
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