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Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.

Abstract:

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required a...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.dib.2016.06.033

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Role:
Author
ORCID:
0000-0002-7249-4762
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Cancer Research UK More from this funder
Danish Cancer Society More from this funder
Cambridge University More from this funder
Prostate Cancer UK More from this funder
Norwegian Cancer Society More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Data in Brief Journal website
Volume:
8
Pages:
733-740
Publication date:
2016-06-01
Acceptance date:
2016-06-21
DOI:
EISSN:
2352-3409
ISSN:
2352-3409
Pmid:
27508226
Source identifiers:
817476

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