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Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.

Abstract:

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required a...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.dib.2016.06.033

Authors


Temmerman, K More by this author
Lawrence-Dörner, A-M More by this author
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ORCID:
0000-0002-7249-4762
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Norwegian Research Council More from this funder
Southern and Eastern Norway Regional Health Authority More from this funder
University of Oslo More from this funder
Centre for Molecular Medicine Norway More from this funder
Cancer Research UK More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Data in Brief Journal website
Volume:
8
Pages:
733-740
Publication date:
2016-06-05
Acceptance date:
2016-06-21
DOI:
EISSN:
2352-3409
ISSN:
2352-3409
Pubs id:
pubs:817476
URN:
uri:4c515941-c921-4c60-8fdc-0f4317f45aa7
UUID:
uuid:4c515941-c921-4c60-8fdc-0f4317f45aa7
Local pid:
pubs:817476

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