- Abstract:
-
Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required a...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.2mb)
-
- Publisher copy:
- 10.1016/j.dib.2016.06.033
-
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- Publisher:
- Elsevier Publisher's website
- Journal:
- Data in Brief Journal website
- Volume:
- 8
- Pages:
- 733-740
- Publication date:
- 2016-06-05
- Acceptance date:
- 2016-06-21
- DOI:
- EISSN:
-
2352-3409
- ISSN:
-
2352-3409
- Pubs id:
-
pubs:817476
- URN:
-
uri:4c515941-c921-4c60-8fdc-0f4317f45aa7
- UUID:
-
uuid:4c515941-c921-4c60-8fdc-0f4317f45aa7
- Local pid:
- pubs:817476
- Language:
- English
- Keywords:
- Copyright holder:
- Crown Copyright
- Copyright date:
- 2016
- Notes:
- Crown Copyright © 2016 Published by Elsevier Inc. This is an open access article under the CCBY license (http://creativecommons.org/licenses/by/4.0/).
Journal article
Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli.
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Norwegian Research Council
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Southern and Eastern Norway Regional Health Authority
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University of Oslo
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Centre for Molecular Medicine Norway
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Cancer Research UK
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