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Measurement of specific [3H]-ouabain binding to different types of human leucocytes.

Abstract:

We have studied the specific binding of [3H]-ouabain to intact mononuclear leucocytes (82% lymphocytes) and polymorphonuclear leucocytes. In both types of cells [3H]-ouabain binding was saturable, confined to a single site of high affinity, slow to reach equilibrium, slow to reverse, temperature-dependent, competitively antagonized by potassium, and facilitated by the presence of divalent cations. The equilibrium dissociation constants were 2.4 +/- 0.7 nmol/l (polymorphs) and 2.4 +/- 0.4 nmol...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Primary Care Health Sciences, PHC-Seniors
Role:
Author
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Journal:
British journal of clinical pharmacology
Volume:
18
Issue:
2
Pages:
153-161
Publication date:
1984-08-05
DOI:
EISSN:
1365-2125
ISSN:
0306-5251
URN:
uuid:4c28d124-05d7-47b4-8fac-eba43ae94114
Source identifiers:
322155
Local pid:
pubs:322155

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