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Quantitative characterization of cell transduction by HSV-1 amplicons using flow cytometry and real-time PCR.

Abstract:

Herpes simplex virus type 1 (HSV-1) amplicon preparations are usually quantified as transducing units/ml (TU/ml), with little information on genomic copy/TU ratios. In the present study, two HSV-1 amplicons expressing enhanced green fluorescent protein (EGFP) were analysed by quantitative PCR (qPCR) and transducing activity to obtain genomic copy/TU ratios. One vector (pHSV-GL) contains the HSV-1 packaging signal (pac) and origin of replication (oriS) and the other (pHSV/EBV-GL) includes Epst...

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Publication status:
Published

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Authors


El-Sherbini, YM More by this author
Stevenson, MM More by this author
Seymour, LW More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Journal:
Journal of virological methods
Volume:
159
Issue:
2
Pages:
160-166
Publication date:
2009-08-05
DOI:
EISSN:
1879-0984
ISSN:
0166-0934
URN:
uuid:4b79b165-2764-4012-baed-150b0f3884cb
Source identifiers:
113826
Local pid:
pubs:113826

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