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Screening for Novel Endogenous Inflammatory Stimuli Using the Secreted Embryonic Alkaline Phosphatase NF-κB Reporter Assay

Abstract:

An immune response can be activated by pathogenic stimuli, as well as endogenous danger signals, triggering the activation of pattern recognition receptors and initiating signalling cascades that lead to inflammation. This method uses THP1-BlueTM cells, a human monocytic cell line which contains an embryonic alkaline phosphatase reporter gene allowing the detection of NF-κB-induced transcriptional activation. We validated this protocol by assessing NF-κB activation after stimulation of toll-l...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.21769/BioProtoc.2220

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS, KIR
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS, KIR
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS, KIR
Arthritis Research UK More from this funder
Kennedy Trust for Rheumatology Research More from this funder
Publisher:
Bio-protocol LLC Publisher's website
Journal:
Bioprotocols Journal website
Volume:
7
Issue:
7
Publication date:
2017-04-05
Acceptance date:
2017-02-27
DOI:
ISSN:
2331-8325
Pubs id:
pubs:688396
URN:
uri:4ae8e037-2122-40f3-8b04-82274e5024a9
UUID:
uuid:4ae8e037-2122-40f3-8b04-82274e5024a9
Local pid:
pubs:688396

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