N.m.r. determination of the solution conformation and dynamics of the A.G mismatch in the d(CGCAAATTGGCG)2 dodecamer.

Journal article

A.G base-paired mismatches that occur during replication are among the most difficult to detect by repair enzymes. Such purine.purine mispairs can exist in two conformations, one of which is stabilized by protons [Gao and Patel (1988) J. Am. Chem. Soc. 110, 5178-5182]. We have undertaken a 1H-n.m.r. and 31P-n.m.r. study of the mismatched dodecamer d(CGCAAATTGGCG)2 as a function of both temperature and pH to determine the conformational features of the A.G mismatch. At pH greater than 7 the mi...

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Authors: Lane, A; Jenkins, T; Brown, D; Brown, T

• Publication status: Published
• Journal: Biochemical journal
• Volume: 279 ( Pt 1)
• Pages: 269-281
• Publication date: 1991-10-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Electricity; Models, Molecular; Thermodynamics; Nucleic Acid Hybridization; Nucleic Acid Heteroduplexes; Base Sequence; Magnetic Resonance Spectroscopy; DNA; Kinetics; Molecular Sequence Data; Hydrogen-Ion Concentration