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Gamma-H2AX foci counting: Image processing and control software for high-content screening

Abstract:

Phosphorylation of the chromatin protein H2AX (forming γH2AX) is implicated in the repair of DNA double strand breaks (DSB's); a large number of H2AX molecules become phosphorylated at the sites of DSB's. Fluorescent staining of the cell nuclei for γH2AX, via an antibody, visualises the formation of these foci, allowing the quantification of DNA DSB's and forming the basis for a sensitive biological dosimeter of ionising radiation. We describe an automated fluorescence microscopy system, incl...

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Publisher copy:
10.1117/12.705217

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Institution:
University of Oxford
Department:
Oxford, MSD, Oncology
Role:
Author
Journal:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume:
6441
Publication date:
2007-01-01
DOI:
ISSN:
1605-7422
URN:
uuid:4ad2933b-8139-4d16-a295-8ce54695c67e
Source identifiers:
132874
Local pid:
pubs:132874

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