Analysis of a deep transcriptome from the mantle tissue of Patella vulgata Linnaeus (Mollusca: Gastropoda: Patellidae) reveals candidate biomineralising genes.

Journal article

The gastropod Patella vulgata is abundant on rocky shores in Northern Europe and a significant grazer of intertidal algae. Here we report the application of Illumina sequencing to develop a transcriptome from the adult mantle tissue of P. vulgata. We obtained 47,237,104 paired-end reads of 51 bp, trialled de novo assembly methods and settled on the additive multiple K method followed by redundancy removal as resulting in the most comprehensive assembly. This yielded 29,489 contigs of at least...

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Authors: Werner, G; Gemmell, P; Grosser, S; Hamer, R; Shimeld, S

• Publication status: Published
• Journal: Marine biotechnology (New York, N.Y.)
• Volume: 15
• Issue: 2
• Pages: 230-243
• Publication date: 2013-04-01
• DOI: 10.1007/s10126-012-9481-0
• EISSN: 1436-2236
• ISSN: 1436-2228
• Language: English
• Keywords: Tandem Repeat Sequences; Minerals; Molecular Sequence Data; Sequence Analysis, RNA; Computational Biology; Reverse Transcriptase Polymerase Chain Reaction; Gastropoda; Gene Expression Profiling; Genetic Association Studies; Models, Genetic; England; RNA, Messenger; DNA Primers; Animal Shells; Animals; Transcriptome; Base Sequence; In Situ Hybridization