Baculovirus as delivery system for gene transfer during hypothermic organ preservation.

Journal article

Concerns over the safety of conventional viral vectors have limited the translation of gene transfer from an exciting experimental procedure to a successful clinical therapy in transplantation. Baculoviruses are insect viruses, but have the ability to enter mammalian cells and deliver potential therapeutic molecules with no evidence of viral replication. This study provides evidence of the ability of recombinant baculovirus to enter mammalian kidneys and livers during cold preservation. Six k...

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Authors: Murguía-Meca, F; Plata-Muñoz, J; Hitchman, R; Danquah, J; Hughes, D

• Publication status: Published
• Journal: Transplant international : official journal of the European Society for Organ Transplantation
• Volume: 24
• Issue: 8
• Pages: 820-828
• Publication date: 2011-08-01
• DOI: 10.1111/j.1432-2277.2011.01252.x
• EISSN: 1432-2277
• ISSN: 0934-0874
• Language: English
• Keywords: Adenosine Triphosphate; Microscopy, Confocal; Hypothermia, Induced; Gene Transfer Techniques; Allopurinol; Organ Preservation Solutions; Genomics; Time Factors; Green Fluorescent Proteins; Baculoviridae; Organ Preservation; Humans; Luminescent Proteins; Genetic Vectors; Proteomics; RNA, Messenger; Animals; Glutathione; Raffinose; Insulin; Swine; Cell Survival; Female; Adenosine