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Adsorption of serum albumins at the air/water interface

Abstract:

The adsorption of bovine serum albumin and human serum albumin (HSA) at the air/water interface has been studied by neutron specular reflection. All the neutron measurements were performed in null reflecting water (D2O:H2O≈1:11), and the specular reflectivity at this water contrast is entirely from the adsorbed protein layer at the air/water interface. Accurate measurement of surface excesses and layer thicknesses has allowed us to infer the possible structural conformation of the two protein...

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Publication status:
Published

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Publisher copy:
10.1021/la990131h

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Publisher:
ACS
Journal:
LANGMUIR
Volume:
15
Issue:
20
Pages:
6975-6983
Publication date:
1999-09-28
DOI:
EISSN:
1520-5827
ISSN:
0743-7463
URN:
uuid:4a4911e9-0984-44a9-af0d-e356f09fa7b4
Source identifiers:
121651
Local pid:
pubs:121651
Language:
English

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