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The controlled introduction of multiple negative charge at single amino acid sites in subtilisin Bacillus lentus.

Abstract:

The use of methanethiosulfonates as thiol-specific modifying reagents in the strategy of combined site-directed mutagenesis and chemical modification allows virtually unlimited opportunities for creating new protein surface environments. As a consequence of our interest in electrostatic manipulation as a means of tailoring enzyme activity and specificity, we have adopted this approach for the controlled incorporation of multiple negative charges at single sites in the representative serine pr...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
DeSantis, G More by this author
Journal:
Bioorganic and medicinal chemistry
Volume:
7
Issue:
11
Pages:
2293-2301
Publication date:
1999-11-05
DOI:
EISSN:
1464-3391
ISSN:
0968-0896
URN:
uuid:48a82406-edaf-415d-8329-df04aaee05c2
Source identifiers:
52040
Local pid:
pubs:52040

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