Selective inhibition of human type 1 11beta-hydroxysteroid dehydrogenase by synthetic steroids and xenobiotics.

Journal article

Functional analyses were performed with microsomal human 11beta-hydroxysteroid dehydrogenase type 1 overexpressed in the yeast Pichia pastoris. Cell extracts or microsomes from transformed strains displayed dehydrogenase and reductase activities, which were up to 10 times higher than in human liver microsomes, while for whole cells cortisone reduction but no dehydrogenase activity was observed. The synthetic glucocorticoids prednisolone and prednisone were efficiently metabolized by subcellul...

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Authors: Hult, M; Jörnvall, H; Oppermann, U

• Journal: FEBS letters
• Volume: 441
• Issue: 1
• Pages: 25-28
• Publication date: 1998-12-01
• DOI: 10.1016/s0014-5793(98)01515-4
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: 11-beta-Hydroxysteroid Dehydrogenases; Steroids; Kinetics; Microsomes; Recombinant Proteins; Xenobiotics; Furosemide; Substrate Specificity; Flavanones; Enzyme Inhibitors; Pichia; Hydroxysteroid Dehydrogenases; Humans; Flavonoids; Cloning, Molecular; Estradiol Congeners