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Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage.

Abstract:

Nuclear DNA is organised into loops, probably by attachment to a supramolecular structure. We describe a method which enables us to map the position of sequences within a loop relative to the point of attachment. Nuclear DNA is isolated unbroken by lysing HeLa cells in 2M NaCl to release structures which retain many of the morphological features of nuclei. Their DNA is supercoiled and so must remain unbroken and looped during lysis. Nucleoids are digested to various degrees with a restriction...

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Publication status:
Published

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Publisher copy:
10.1093/nar/8.13.2895

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Brazell, IA More by this author
Journal:
Nucleic acids research
Volume:
8
Issue:
13
Pages:
2895-2906
Publication date:
1980-07-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:472ee39d-430e-4bbd-bb19-cec76e5e1e8f
Source identifiers:
7027
Local pid:
pubs:7027

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