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2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation.

Abstract:

The phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistanc...

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Publication status:
Published

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Publisher copy:
10.1002/chem.201102287

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Journal:
Chemistry (Weinheim an der Bergstrasse, Germany)
Volume:
17
Issue:
52
Pages:
14851-14856
Publication date:
2011-12-05
DOI:
EISSN:
1521-3765
ISSN:
0947-6539
URN:
uuid:471f2e67-25d8-40fa-9006-425f587c1b3d
Source identifiers:
399986
Local pid:
pubs:399986

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