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Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors.

Abstract:

Three strategies have been designed to concentrate infectious retroviral vectors from the supernatants of human- (HT1080) and murine- (NIH 3T3) based packaging cells. Streptavidin-conjugated paramagnetic particles in conjunction with (i) antibodies directed against murine fibronectin, (ii) biotinylated lectins, or (iii) biotin-modified packaging cell-surface proteins allow affinity-mediated magnetic concentration of retroviral vectors. Retroviral titers (assayed by colony formation of human m...

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Publication status:
Published

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Publisher copy:
10.1006/mthe.2001.0268

Authors


Journal:
Molecular therapy : the journal of the American Society of Gene Therapy
Volume:
3
Issue:
4
Pages:
623-630
Publication date:
2001-04-05
DOI:
EISSN:
1525-0024
ISSN:
1525-0016
URN:
uuid:4698955f-9e47-46e4-8418-f87098ec47a4
Source identifiers:
466390
Local pid:
pubs:466390

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