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Spectrofluorometric measurement of the binding of ethidium to superhelical DNA from cell nuclei.

Abstract:

Structures retaining many of the morphological features of nuclei may be released by lysing HeLa cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain few chromatin proteins. We have shown that the DNA of nucleoids is quasicircular and supercoiled by measure spectrofluorometrically the amount of the intercalating dye, ethidium, bound to unirradiated and gamma-irradiated nucleoids. Ethidium binds to nucleoids in the manner characteristic of...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
European journal of biochemistry / FEBS
Volume:
84
Issue:
2
Pages:
465-477
Publication date:
1978-03-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:4682c289-6c0f-461f-8f82-de5c76783f59
Source identifiers:
25434
Local pid:
pubs:25434

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