- Abstract:
- α-Mannosides made easy: Mutation of a family-GH31 α-glucosidase that displays plasticity to alterations at the 2-OH position of donor substrates created an efficient α-mannoside-synthesizing biocatalyst. A simple fluoride donor reagent was used for the synthesis of a range of mono-α-mannosylated conjugates using the α-mannosynthase displaying low (unwanted) oligomerization activity. Copyright © 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.
- Publication status:
- Published
- Publisher copy:
- 10.1002/anie.201201081
-
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- Journal:
- Angewandte Chemie (International ed. in English)
- Volume:
- 51
- Issue:
- 30
- Pages:
- 7449-7453
- Publication date:
- 2012-07-05
- DOI:
- EISSN:
-
1521-3773
- ISSN:
-
1433-7851
- URN:
-
uuid:459a8a18-01a3-4adb-bf45-d1ba1934bc49
- Source identifiers:
-
339834
- Local pid:
- pubs:339834
- Language:
- English
- Keywords:
- Copyright date:
- 2012
Journal article
Creation of an α-mannosynthase from a broad glycosidase scaffold.
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