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Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.

Abstract:
Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

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Authors


Nettleship, JE More by this author
Watson, PJ More by this author
Rahman-Huq, N More by this author
Fairall, L More by this author
Posner, MG More by this author
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Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
1258
Pages:
209-222
Publication date:
2015-01-05
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:4563ab79-6e24-4716-8e5a-778cab655e15
Source identifiers:
493456
Local pid:
pubs:493456

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