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Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)

Abstract:

By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial ...

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Publisher copy:
10.14411/eje.2003.070

Authors


Macours, N More by this author
Vandingenen, A More by this author
Gielens, C More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Baggerman, G More by this author
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Journal:
European Journal of Entomology
Volume:
100
Issue:
4
Pages:
467-474
Publication date:
2003
DOI:
EISSN:
1802-8829
ISSN:
1210-5759
URN:
uuid:45277b3d-6abb-4ff1-982c-b19185cfbea2
Source identifiers:
349406
Local pid:
pubs:349406

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