Depolarization-independent net uptake of calcium into clonal insulin-releasing cells exposed to glucose.

Journal article

Insulin release, net fluxes of Ca2+, and glucose metabolism were studied in a clonal cell line (RINm5F) established from a transplantable rat islet tumor. The insulin content amounted to only 0.03% of that of the total protein and decreased even further with subsequent passages. The insulin secretion was as high as 10 to 20% of the total hormone content per hour. Insulin release was stimulated by K+ depolarization but not by exposure to glucose. In contrast to this secretory pattern, glucose but not K+ stimulated the net uptake of Ca2+ at micromolar concentrations of the ion. The glucose effect was not mimicked by 20 mM 3-O-methylglucose. It was as pronounced at 1 mM as at 20 mM of the sugar and corresponded to an uptake of 119 fmol cm-2 s-1. Glucose metabolism was typical for tumor cells with a high glycolytic flux and an oxidation-to-utilization ratio as low as 0.05-0.15. Maximal oxidative degradation was attained already at 1 mM. This concentration was also equivalent to the Km for glucose utilization, indicating a substantial left-hand shift of the normal dose-response curve. It is suggested that glucose induces a depolarization-independent net uptake of Ca2+ by favouring intracellular buffering of the cation.

Authors: Gylfe, E; Andersson, T; Rorsman, P; Abrahamsson, H; Arkhammar, P

• Publication status: Published
• Publisher: Kluwer Academic Publishers
• Journal: Bioscience reports
• Volume: 3
• Issue: 10
• Pages: 927-937
• Publication date: 1983-10-01
• DOI: 10.1007/bf01140662
• EISSN: 1573-4935
• ISSN: 0144-8463
• Language: English
• Keywords: Pancreatic Neoplasms; Animals; Potassium; Calcium; Rats; Insulin; Glucose; Clone Cells; Adenoma, Islet Cell; Biological Transport, Active