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Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes.

Abstract:

The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the gamma-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was approximately 50, approximately 25, and approximately 4% respectively....

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Publication status:
Published

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Publisher copy:
10.1007/s10863-007-9114-x

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Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Role:
Author
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Journal:
Journal of bioenergetics and biomembranes
Volume:
39
Issue:
5-6
Pages:
435-439
Publication date:
2007-12-01
DOI:
EISSN:
1573-6881
ISSN:
0145-479X
Source identifiers:
155076
Language:
English
Keywords:
Pubs id:
pubs:155076
UUID:
uuid:43b09b1a-f8b0-4f3f-a3cd-f11f230ca7f6
Local pid:
pubs:155076
Deposit date:
2013-11-16

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