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The infectious BAC genomic DNA expression library: a high capacity vector system for functional genomics

Abstract:

Gene dosage plays a critical role in a range of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors which express proteins well above physiological levels. In contrast, genomic DNA expression vectors generate physiologically-relevant levels of gene expression by carrying the whole genomic DNA locus of a gene including its regulatory elements. Here we describe the first genomic DNA expression library generated using the high-capacity herpes simplex vir...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/srep28644

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Edser, PAH More by this author
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Funding agency for:
Lufino, MMP
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Funding agency for:
Wade-Martins, R
Cancer Research UK More from this funder
Wellcome Trust More from this funder
Publisher:
Nature Publishing Group: Open Access Journals - Option C Publisher's website
Journal:
PLoS ONE Journal website
Volume:
6
Pages:
Article: 28644
Publication date:
2016-06-29
Acceptance date:
2016-05-13
DOI:
ISSN:
2045-2322
URN:
uuid:43618ae5-ae88-4fa7-8c04-59fcc7a00ae4
Source identifiers:
629473
Local pid:
pubs:629473

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