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Journal article

Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.

Abstract:

The measurement of changes in nicotinic acid adenine dinucleotide phosphate (NAADP) levels in cells has been, and remains, key to the investigation of the functions of NAADP as a Ca2+ -releasing second messenger. Here we provide details of how to isolate NAADP from cells by extraction with perchloric acid and then measure the NAADP using a radioreceptor assay. We demonstrate that NAADP is neither generated nor broken down during sample processing conditions and that radioreceptor assay is hig...

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Publication status:
Published

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Publisher copy:
10.1016/j.ab.2007.08.030

Authors


Masgrau, R More by this author
Vasudevan, SR More by this author
Yamasaki, M More by this author
O'Neill, JS More by this author
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Journal:
Analytical biochemistry
Volume:
371
Issue:
1
Pages:
26-36
Publication date:
2007-12-05
DOI:
EISSN:
1096-0309
ISSN:
0003-2697
URN:
uuid:43236c7a-0094-494b-a219-0f466d42c33d
Source identifiers:
106488
Local pid:
pubs:106488

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