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The genomic characterization of Salmonella Paratyphi A from an outbreak of enteric fever in Vadodara, India

Abstract:

Salmonella enterica Typhi (S. Typhi) and Paratyphi A (S. Paratyphi A) are the causative agents of enteric fever, a systemic human disease with a burden of 300 000 cases per year in India. The majority of enteric fever cases are associated with S. Typhi, resulting in a paucity of data regarding S. Paratyphi A, specifically with respect to genomic surveillance and antimicrobial resistance (AMR). Here, we exploited whole-genome sequencing (WGS) to identify S. Paratyphi A genotypes and AMR determinants associated with an outbreak of S. Paratyphi A in Vadodara, India, from December 2018 to December 2019. In total 117 S. Paratyphi A were isolated and genome sequenced, most were genotype 2.4.2 (72.6 % of all cases), which is the globally dominant genotype. The remainder were genotype 2.3 (25.6 %), while only two isolates belonged to genotype 2.4.1. A single base-pair mutation in gyrA, associated with reduced susceptibility to fluoroquinolones, was present in all of the outbreak isolates; with 74.35 % of isolates having a S83F substitution and the remainder having an S83Y substitution. Our surveillance study suggests that S. Paratyphi A is an emergent pathogen in South Asia, which may become increasingly relevant with the introduction of Vi conjugate vaccines.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1099/mgen.0.000914

Authors


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Role:
Author
ORCID:
0000-0001-8456-5874


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Funder identifier:
https://ror.org/029chgv08
Grant:
215515/Z/19/Z


Publisher:
Microbiology Society
Journal:
Microbial Genomics More from this journal
Volume:
9
Issue:
1
Article number:
000914
Publication date:
2023-01-06
Acceptance date:
2022-10-18
DOI:
EISSN:
2057-5858
Pmid:
36748526


Language:
English
Keywords:
Pubs id:
1329707
Local pid:
pubs:1329707
Deposit date:
2025-01-14

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