Journal article icon

Journal article

Ratiometric fluorescence-imaging assays of plant membrane traffic using polyproteins.

Abstract:

Fluorescent protein markers are widely used to report plant membrane traffic; however, effective protocols to quantify fluorescence or marker expression are lacking. Here the 20 residue self-cleaving 2A peptide from Foot and Mouth Disease Virus was used to construct polyproteins that expressed a trafficked marker in fixed stoichiometry with a reference protein in a different cellular compartment. Various pairs of compartments were simultaneously targeted. Together with a bespoke image analysi...

Expand abstract
Publication status:
Published

Actions


Access Document


Authors


Samalova, M More by this author
Fricker, M More by this author
Journal:
Traffic (Copenhagen, Denmark)
Volume:
7
Issue:
12
Pages:
1701-1723
Publication date:
2006-12-05
DOI:
EISSN:
1600-0854
ISSN:
1398-9219
URN:
uuid:425755ea-84cc-485b-a49d-854f59e4e13d
Source identifiers:
48870
Local pid:
pubs:48870

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP