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Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells.

Abstract:

In this chapter, protocols are described for converting mouse monoclonal antibodies into recombinant Fabs for transient expression in mammalian cells. Variable region genes are cloned by reverse transcription: PCR using either sequence specific or mixed 5' primers that hybridise to the first framework sequence of the mouse light and heavy chains and 3' primers that bind to the heavy- and light-chain constant regions. The amplified sequences are inserted into mammalian cell expression vectors ...

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
801
Pages:
137-159
Publication date:
2012-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
186655

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