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Structure and expression of an alpha-tubulin gene of Physarum polycephalum.

Abstract:

Fragments of Physarum polycephalum DNA generated by partial digestion with Sau3A were cloned into phage-lambda EMBL4. A recombinant (phage-lambda E alpha Tu) containing an alpha-tubulin (E alpha-tubulin) gene was isolated. The E alpha-tubulin gene is part of the alt B locus. The gene was sequenced and was found to contain seven intervening sequences. The alpha-tubulin isotype (E alpha-tubulin) encoded by the gene has a methionine residue at the C-terminus. The E alpha-tubulin gene has much in...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
European journal of biochemistry / FEBS
Volume:
181
Issue:
3
Pages:
583-592
Publication date:
1989-05-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:41b73a2f-879c-4ac4-abec-20dee3508ad4
Source identifiers:
13029
Local pid:
pubs:13029

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