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Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy.

Abstract:

Experiments based on fluorescence detection are limited by the population of the fluorescence marker's long-lived dark triplet state, leading to pronounced photobleaching reactions and blinking which reduces the average fluorescence signal obtained per time interval. By irradiation with a second, red-shifted laser line, we initiate reverse intersystem crossing (ReISC) which enhances the fluorescence signal of common fluorophores up to a factor of 14. The reverse intersystem crossing from the ...

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Publication status:
Published

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Publisher copy:
10.1002/cphc.200700596

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Journal:
Chemphyschem : a European journal of chemical physics and physical chemistry
Volume:
9
Issue:
4
Pages:
612-624
Publication date:
2008-03-05
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:41675733-e649-4f7f-9800-fc12490af36d
Source identifiers:
222184
Local pid:
pubs:222184

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