Rapid actions of 17beta-oestradiol on a subset of lactotrophs in the rat pituitary.

Journal article

Increasingly the role of rapid mechanisms of steroid action in physiological regulation are being recognised. We have investigated rapid effects of 17beta-oestradiol (E) on prolactin (PRL) release in vitro. Pituitary segments from male rats were incubated for 5, 10 or 20 min in Earle's balanced salt solution containing 1.2 mM tannic acid (to enable visualisation of exocytosed secretory granules by electron microscopy) either alone (control) or containing 10(-10)-10(-8) M E conjugated to bovin...

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Authors: Christian, H; Morris, J

• Publication status: Published
• Journal: Journal of physiology
• Volume: 539
• Issue: Pt 2
• Pages: 557-566
• Publication date: 2002-03-01
• DOI: 10.1113/jphysiol.2001.012947
• EISSN: 1469-7793
• ISSN: 0022-3751
• Language: English
• Keywords: Radioimmunoassay; Serum Albumin, Bovine; Luteinizing Hormone; Hydrolyzable Tannins; Animals; Rats, Sprague-Dawley; Receptors, Cell Surface; Flow Cytometry; Microscopy, Electron; Female; Male; Antimetabolites; Pituitary Gland, Anterior; Estradiol; Exocytosis; Receptors, Estrogen; Rats; Prolactin; Cyclic AMP