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Residual dipolar couplings: are multiple independent alignments always possible?

Abstract:

RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the protein's interaction with some alignment media increases the experimental error. Together with heterogeneity across the alignment media arising from constraints on temperature, pH and ionic strength for so...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/s10858-010-9457-1

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Funding agency for:
Redfield, C
Grant:
079440
Publisher:
Springer Publisher's website
Journal:
Journal of Biomolecular NMR Journal website
Volume:
49
Issue:
1
Pages:
53-60
Publication date:
2011-01-01
DOI:
EISSN:
1573-5001
ISSN:
0925-2738
Language:
English
Keywords:
Subjects:
UUID:
uuid:41264b56-79e5-4140-9603-256d2995aa23
Local pid:
ora:5468
Deposit date:
2011-06-17

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