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Residual dipolar couplings: are multiple independent alignments always possible?

Abstract:

RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the protein's interaction with some alignment media increases the experimental error. Together with heterogeneity across the alignment media arising from constraints on temperature, pH and ionic strength for so...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1007/s10858-010-9457-1

Authors


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Institution:
University of Oxford
Department:
Medical Sciences Division - Biochemistry
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Institution:
University of Oxford
Department:
Medical Sciences Division - Biochemistry
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Institution:
University of Oxford
Department:
Mathematical,Physical & Life Sciences Division - Chemistry - Inorganic Chemistry Laboratory
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Institution:
University of Oxford
Department:
Medical Sciences Division - Biochemistry
More from this funder
Funding agency for:
Christina Redfield
Publisher:
Springer Publisher's website
Journal:
Journal of Biomolecular NMR Journal website
Volume:
49
Issue:
1
Pages:
53-60
Publication date:
2011
DOI:
EISSN:
1573-5001
ISSN:
0925-2738
URN:
uuid:41264b56-79e5-4140-9603-256d2995aa23
Local pid:
ora:5468

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