Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.

Journal article

Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.

Authors: Kershaw, N; Mukherji, M; MacKinnon, C; Claridge, T; Odell, B

• Publication status: Published
• Journal: Bioorganic and medicinal chemistry letters
• Volume: 11
• Issue: 18
• Pages: 2545-2548
• Publication date: 2001-09-01
• DOI: 10.1016/s0960-894x(01)00494-2
• EISSN: 1464-3405
• ISSN: 0960-894X
• Language: English
• Keywords: Spectrometry, Mass, Electrospray Ionization; Magnetic Resonance Spectroscopy; Recombinant Proteins; Phytanic Acid; Stereoisomerism; Humans; Coenzyme A; Mixed Function Oxygenases