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Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.

Abstract:

The collagen-binding domain of human fibronectin has been expressed as a cro/beta-galactosidase fusion protein in Escherichia coli. The hybrid polypeptide was recognized by an anti-(human plasma fibronectin) serum and bound specifically to gelatin-Sepharose. The collagen-binding region was subdivided by constructing a series of overlapping bacterial expression plasmids. The fusion proteins produced by these constructs were analysed for gelatin-binding activity. The results indicate that the b...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
EMBO journal
Volume:
5
Issue:
11
Pages:
2825-2830
Publication date:
1986-11-01
EISSN:
1460-2075
ISSN:
0261-4189
Source identifiers:
42659
Language:
English
Keywords:
Pubs id:
pubs:42659
UUID:
uuid:40939ca4-3a90-4e03-9dc8-5ac57963d586
Local pid:
pubs:42659
Deposit date:
2012-12-19

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