Journal article
Robust detection of chromosomal interactions from small numbers of cells using low-input Capture-C
- Abstract:
-
Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin architecture in rare cell populations. We present a new low-input Capture-C approach that can generate high-quality 3C interaction profiles from 10 000-20 000 cells, depending on the resolution used for analysis. We also present a PCR-free, sequencing-free 3C techniq...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
Bibliographic Details
- Publisher:
- Oxford University Press Publisher's website
- Journal:
- Nucleic Acids Research Journal website
- Volume:
- 45
- Issue:
- 22
- Article number:
- e184
- Publication date:
- 2017-11-23
- Acceptance date:
- 2017-11-21
- DOI:
- EISSN:
-
1362-4962
- ISSN:
-
0305-1048
- Pmid:
-
29186505
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:809379
- UUID:
-
uuid:404e1171-3977-457a-a305-5afd4c4bf4c8
- Local pid:
- pubs:809379
- Source identifiers:
-
809379
- Deposit date:
- 2018-05-11
Terms of use
- Copyright holder:
- © Oudelaar, et al 2017 Published by Oxford University Press on behalf of Nucleic Acids Research
- Copyright date:
- 2017
- Notes:
- This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited
- Licence:
- CC Attribution (CC BY)
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