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Synthesis and properties of triplex-forming oligonucleotides containing 2'-O-(2-methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine.

Abstract:
2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases.
Publication status:
Published

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Publisher copy:
10.1016/j.bmc.2010.07.005

Authors


Brennan, L More by this author
Vergara-Irigaray, N More by this author
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Journal:
Bioorganic and medicinal chemistry
Volume:
18
Issue:
17
Pages:
6389-6397
Publication date:
2010-09-05
DOI:
EISSN:
1464-3391
ISSN:
0968-0896
URN:
uuid:403112e9-2be4-49c3-bbe7-c83336a8dae7
Source identifiers:
399999
Local pid:
pubs:399999

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