Adenovirus cores can function as templates in in vitro DNA replication.

Journal article

Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein-dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA-protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.

Authors: Goding, C; Russell, W

• Publication status: Published
• Journal: EMBO journal
• Volume: 2
• Issue: 3
• Pages: 339-344
• Publication date: 1983-01-01
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Viral Core Proteins; Novobiocin; DNA Gyrase; Deoxycytidine Monophosphate; Templates, Genetic; Adenoviruses, Human; Enzyme Inhibitors; DNA, Viral; Viral Proteins; Humans; Phosphoproteins; Hela Cells; DNA Replication; Protein Precursors