The ATP- and tolbutamide-sensitivity of the ATP-sensitive K-channel from human pancreatic B cells.

Journal article

The ATP- and sulphonylurea-sensitivity of the ATP-sensitive K-channel was measured in human pancreatic B cells. In inside-out patches, half-maximal inhibition of channel activity was produced by 10 mumol/l ATP (with 2 mM Mg2+) and ATP-inhibition was partially antagonised by ADP. A significantly lower sensitivity to ATP was found in whole-cell recordings. Tolbutamide inhibited whole-cell ATP-sensitive K-currents half-maximally at 18 mumol/l; the sensitivity to tolbutamide was somewhat less in ...

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Authors: Ashcroft, F; Kakei, M; Gibson, J; Gray, D; Sutton, R

• Publication status: Published
• Publisher: Springer-Verlag
• Journal: Diabetologia
• Volume: 32
• Issue: 8
• Pages: 591-598
• Publication date: 1989-08-01
• DOI: 10.1007/bf00285333
• EISSN: 1432-0428
• ISSN: 0012-186X
• Language: English
• Keywords: Islets of Langerhans; Kinetics; Potassium Channels; Tolbutamide; Membrane Potentials; Calcium; Adenosine Triphosphate; Humans