This paper considers problems associated with the accurate determination of enzyme activity using coupled spectrophotometric assays. Criteria for establishing optimum assay conditions and ensuring that the coupled assay accurately reflects enzyme activity are presented. The susceptibility of such assays to interference is illustrated by five instances in which contamination of specific assay components has produced misleading estimates of phosphofructokinase and pyrophosphate:fructose 6-phosp...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Errors and artifacts in coupled spectrophotometric assays of enzyme activity.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record