Nucleobase recognition in ssDNA at the central constriction of the alpha-hemolysin pore.

Journal article

Nanopores are under investigation for single-molecule DNA sequencing. The alpha-hemolysin (alphaHL) protein nanopore contains three recognition points capable of nucleobase discrimination in individual immobilized ssDNA molecules. We have modified the recognition point R(1) by extensive mutagenesis of residue 113. Amino acids that provide an energy barrier to ion flow (e.g., bulky or hydrophobic residues) strengthen base identification, while amino acids that lower the barrier weaken it. Amin...

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Authors: Stoddart, D; Heron, A; Klingelhoefer, J; Mikhailova, E; Maglia, G

• Publication status: Published
• Journal: Nano letters
• Volume: 10
• Issue: 9
• Pages: 3633-3637
• Publication date: 2010-09-01
• DOI: 10.1021/nl101955a
• EISSN: 1530-6992
• ISSN: 1530-6984
• Language: English
• Keywords: DNA, Single-Stranded; Models, Molecular; Base Sequence; Mutagenesis; Amino Acid Substitution; Hemolysin Proteins