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Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta

Abstract:

Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes. Identification by mass spectrometry revealed labeling of a wide range...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41598-024-60333-x

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Institution:
University of Oxford
Role:
Author
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Institution:
University of Oxford
Division:
HUMS
Department:
Classics Faculty
Sub department:
Chemistry Research Laboratory
Role:
Author
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Institution:
University of Oxford
Role:
Author
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Institution:
University of Oxford
Role:
Author
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Funder identifier:
https://ror.org/0472cxd90
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Funder identifier:
https://ror.org/018mejw64
Publisher:
Nature Research
Journal:
Scientific Reports More from this journal
Volume:
14
Issue:
1
Article number:
10091
Publication date:
2024-05-02
Acceptance date:
2024-04-22
DOI:
EISSN:
2045-2322
Language:
English
Keywords:
Source identifiers:
1938978
Deposit date:
2024-07-20

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