Naturally occurring glucokinase mutations are associated with defects in posttranslational S-nitrosylation.

Journal article

Posttranslational activation of glucokinase (GCK) through S-nitrosylation has been recently observed in the insulin-secreting pancreatic beta-cell; however, the function of this molecular mechanism in regulating the physiology of insulin secretion is not well understood. To more fully understand the function of posttranslational regulation of GCK, we examined two naturally occurring GCK mutations that map to residues proximal to the S-nitrosylated cysteine and cause mild fasting hyperglycemia...

Expand abstract

Authors: Ding, S; Tribble, N; Kraft, C; Markwardt, M; Gloyn, A

• Journal: Molecular endocrinology (Baltimore, Md.)
• Volume: 24
• Issue: 1
• Pages: 171-177
• Publication date: 2010-01-01
• DOI: 10.1210/me.2009-0138
• EISSN: 1944-9917
• ISSN: 0888-8809
• Language: English
• Keywords: Diabetes Mellitus, Type 2; Insulin; Transfection; Point Mutation; Glucokinase; Humans; Age of Onset; Glucose; Fluorescence Resonance Energy Transfer; Cell Line, Tumor; Animals; Recombinant Fusion Proteins; Enzyme Activation; Insulin-Secreting Cells; Protein Processing, Post-Translational; Kinetics; Luminescent Proteins; Mutant Proteins; Mice; Molecular Imaging