Activation of a fibroblast-specific enhancer of the proalpha2(I) collagen gene in tight-skin mice.

Journal article

OBJECTIVE: Reporter transgenes were introduced into the type 1 tight-skin (Tsk1/+) mouse model of scleroderma to test the hypothesis that fibroblast-specific genetic programs are activated in fibrosis. METHODS: Transgenes harboring upstream fragments of the 5' flanking region of the mouse proalpha2(I) collagen gene (Col1a2), linked to a 400-bp minimal Col1a2 promoter driving an Escherichia coli beta-galactosidase (LacZ) reporter gene, were introduced into Tsk1/+ mice by breeding. Expression o...

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Authors: Denton, C; Zheng, B; Shiwen, X; Zhang, Z; Bou-Gharios, G

• Publication status: Published
• Journal: Arthritis and rheumatism
• Volume: 44
• Issue: 3
• Pages: 712-722
• Publication date: 2001-03-01
• DOI: 10.1002/1529-0131(200103)44:3<712::aid-anr121>3.0.co;2-1
• EISSN: 1529-0131
• ISSN: 0004-3591
• Language: English
• Keywords: Fibroblasts; Disease Models, Animal; Mice, Transgenic; Scleroderma, Systemic; Gene Expression Regulation; Procollagen; Animals; Mice; Enhancer Elements, Genetic