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The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number.

Abstract:

We have investigated the effect of target copy number on the efficiency of stable transformation of the protozoan parasite Trypanosoma brucei. Using a single strain of the organism, we targeted integrative vectors to several different genomic sequences, occurring at copy numbers ranging from 1 to approximately 30,000 per diploid genome, and undertook a systematic assessment of both transformation and integration efficiencies. Even over this vast copy number range, frequency of gene targeting ...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkg445

Authors


Wickstead, B More by this author
Ersfeld, K More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Nucleic acids research
Volume:
31
Issue:
14
Pages:
3993-4000
Publication date:
2003-07-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:3f80d65f-3214-42e1-9f04-60aa0fa9a231
Source identifiers:
14949
Local pid:
pubs:14949

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