Journal article icon

Journal article

Bypassing the proline/thiazoline requirement of the macrocyclase PatG

Abstract:

Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified tw...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

Actions


Access Document


Files:
Publisher copy:
10.1039/c7cc06550g

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author
More from this funder
Funding agency for:
Naismith, J
Grant:
1000 talent scholar
More from this funder
Funding agency for:
Naismith, J
Grant:
1000 talent scholar
More from this funder
Grant:
UK National Mass Spectrometry Facility at Swansea University
Expand funders...
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Communications Journal website
Volume:
53
Issue:
91
Pages:
12274-12277
Publication date:
2017-11-01
Acceptance date:
2017-09-13
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Pmid:
29090689
Source identifiers:
744846
Language:
English
Pubs id:
pubs:744846
UUID:
uuid:3f526e78-5462-4307-a5b6-b70e9cdbfae7
Local pid:
pubs:744846
Deposit date:
2017-11-15

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP