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Bypassing the proline/thiazoline requirement of the macrocyclase PatG

Abstract:

Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified tw...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1039/c7cc06550g

Authors


Stevenson, H More by this author
Jaspars, M More by this author
Westwood, NJ More by this author
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Institution:
University of Oxford
Division:
Medical Sciences Division
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Funding agency for:
Naismith, JH
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Funding agency for:
Naismith, JH
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Grant:
TripleTOF 5600 mass spectrometer (094476), the MALDI TOF–TOF Analyser (079272AIA), 700 NMR
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Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chemical Communications Journal website
Volume:
53
Issue:
91
Pages:
12274-12277
Publication date:
2017-11-01
Acceptance date:
2017-09-13
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Pubs id:
pubs:744846
URN:
uri:3f526e78-5462-4307-a5b6-b70e9cdbfae7
UUID:
uuid:3f526e78-5462-4307-a5b6-b70e9cdbfae7
Local pid:
pubs:744846
Language:
English

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