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Development and characterization of polyspecific anti-mitochondrion antibodies for proteomics studies on in toto tissue homogenates.

Abstract:

We describe the characterization of polyclonal antibodies directed against the whole mitochondrial subproteome, as obtained by hyperimmunization of rabbits with an organelle fraction purified from human skeletal muscle and lysed by sonication. After 2-DE separations with either blue native electrophoresis or IPG as first dimension and blotting, the polyspecific antibodies detect 113 proteins in human muscle mitochondria, representative of all major biochemical pathways and oxidative phosphory...

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Publication status:
Published

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Publisher copy:
10.1002/elps.200800576

Authors


Gianazza, E More by this author
Cazzola, S More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
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Journal:
Electrophoresis
Volume:
30
Issue:
8
Pages:
1329-1341
Publication date:
2009-04-05
DOI:
EISSN:
1522-2683
ISSN:
0173-0835
URN:
uuid:3f3649e9-35b6-47d4-a2c6-2ad67b16e39d
Source identifiers:
230280
Local pid:
pubs:230280

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