Different populations of DNA polymerase alpha in HeLa cells.

Journal article

Three different populations of HeLa DNA polymerase alpha have been distinguished using a novel preparation of chromatin isolated using an isotonic salt concentration, which contains intact DNA. One synthesizes DNA in vitro at 85% of the rate in vivo, is found only in S-phase nuclei tightly associated with the nucleoskeleton and requires unbroken DNA in the form of chromatin as a template: we assume this is the authentic S-phase activity. On incubation at 37 degrees C, this activity dissociate...

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Authors: Jackson, D; Cook, P

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 192
• Issue: 1
• Pages: 77-86
• Publication date: 1986-11-01
• DOI: 10.1016/0022-2836(86)90465-1
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Antibodies; Hela Cells; Humans; DNA Polymerase II; Chromatin; Templates, Genetic; DNA