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Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution.

Abstract:

The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell divisions. The ability to track individual cell lineages is essential for the analysis of gene regulatory factors involved in the control of cell fate and identity decisions. In our approach, cell n...

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Publisher copy:
10.1371/journal.pone.0027886

Authors


Downey, MJ More by this author
Jeziorska, DM More by this author
Koentges, G More by this author
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Journal:
PloS one
Volume:
6
Issue:
12
Pages:
e27886
Publication date:
2011
DOI:
EISSN:
1932-6203
URN:
uuid:3ef9c7ee-b5dd-43de-9fc8-05130f56043a
Source identifiers:
239719
Local pid:
pubs:239719

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